Microsoft Word - 34

Objective of the study is to screen for in vitro pharmacological activities of 5-Lipoxygenase and cytotoxicity of using methanolic callus extracts of Biophytum sensitivum. In vitro Brine shrimp lethality assay was carried out according to the method of Meyer et al., (1982) and the lipoxygenase inhibition activity was assayed using photometric method as described by Reddanna et al., (1985). The callus extract has shown 5-Lipoxygenase potency was found to be low. The Graph pad Prism Version–5 soft ware is used to analyze data in the form of figures. Fenny probed analysis software is used in Brine shrimp lethality assay to obtain ED50 values. But important finding in this study was that the extract has shown significant and remarkable cytotoxicity. The callus extract has shown 5-Lipoxygenase potency was found to be low. This callus extract deserves further evaluation to screen in vivo anti-cancer activity.